Batman (Bruce Wayne):.................................... Kevin Conroy
Robin (Dick Grayson):....................................... Loren Lester
Alfred Pennyworth:............................................ Efrem Zimbalist Jr
Commissioner Gordon:..................................... Bob Hastings
Batgirl/Barbara Gordon:.................................... Melissa Gilbert
The Riddler:....................................................... John Glover
Catwoman (Selina Kyle):................................... Adrienne Barbeau
Maven (Catwoman's Asst.):.............................. Mary McDonald Lewis
The Joker (Jack Napier):................................... Mark Hamill
Mr.Freeze/Dr.Victor Freeze:............................. Michael Ansara
Penguin (Oswald Cobblepot):.......................... Paul Williams
Twoface/Harvey Dent:....................................... Richard Moll
Clayface/Matthew Hagen:................................. Ron Perlman
Kirk Langstrom/Man-Bat:.................................. Marc Singer
The Scarecrow:.................................................. Henry II Polic
Poison Ivy (Dr. Pamela Isley):........................... Diane Pershing
The Mad Hatter:................................................. Roddy McDowall
Detective Bullock:............................................. Robert Costanzo
HARDAC:........................................................... Jeff Bennett
Mayor Hill:.......................................................... Lloyd Bochner
Stern:.................................................................. Herb Edelman
Maddog:............................................................. Greg Burson
Li:........................................................................ Chi Chao
Talia:................................................................... Helen Slater
Killer Croc:......................................................... Aron Kincaid
Dr. Leslie Thompkins:....................................... Diana Muldaur
Harley Quinn:..................................................... Arleen Sorkin
Twitch:................................................................ Chick Vennera
Ronald Daggett:................................................ Ed Asner
Rupert Thorne:................................................. John Vernon
Vertigo:.............................................................. Michael York
Ra's Al Ghul:...................................................... David Warner
Isis the cat:........................................................ Frank Welker
Red Claw:........................................................... Kate Mulgrew
Officer Montoya:............................................... Ingrid Oliu
Shadow Agent:.................................................. Barry Dennen
Summer Gleeson:............................................. Mari Devon
Lucius Fox:........................................................ Brock Peters
Gil Mason:......................................................... Tim Matheson

Other Voices:

Tim Curry
Robert Ito
Buster Jones
Joe Leahy
Tress MacNeille
Neil Ross
Adam West