education

Education and Outreach

EDUCATIONAL PROGRAM:

Biological Training Opportunities: This program focuses on the isolation, preparation, handling and usage of all types of human tissue for flow cytometric evaluation.

Multiple antibodies: Strategies used for staining cells with multiple antibodies simultaneously will be described. The proper verification methods and pitfalls will be discussed. Both antibodies to extracellular and intercellular epitopes will be discussed.
DNA Measurements: An overview of DNA content measurements will be described including the current reagents available and when they should be used. The use of BrDU as a quenching reagent for Hoechst or in combination with a specific antibody including two color labeling with IUDR, will be described.
Cell function: The use of specific molecular probes for evaluating cell function will be discussed. These will include calcium measurements in real time, pH measurements, molecular transfection, mitochondrial and membrane potential dyes and multidrug resistance function.

Flow Cytometry Training: The focus of this program is on multiparameter data acquisition.

Measurement Parameters: A detailed description flow cytometry measurements will be described. This includes FSC, SSC, ALL, fluorescence, polarization, time of flight and fluorescence decay.
Compensation: Theory, practical problems and pitfalls in fluorescence compensation will be discussed. New software methodologies for addressing these problems will be described.
Multiparameter Data Analysis: Instructions on the use of several multiparameter data analysis programs are given. Strategies in pattern recognition will be emphasized. A learning file is initially evaluated to teach the position, problems, and pitfalls for identification of populations. The transfer of this to batch processing of similar data files will be discussed. The concepts of principle components, analysis and clustering will be experienced. Methods for networking PC's, HP's and Macintosh computers together will be explored. The use of mass data storage and archiving will also be discussed.

Pathology Graduate Program: A Masters or Ph.D. program in Experimental Pathology or Immunology is available through the Roswell Park Graduate Division of the State University of New York at Buffalo (SUNYAB). Individuals seeking either program may apply directly to the graduate division and mention flow cytometry as the preferred specialty. Tuition for the Masters and Ph.D. programs can be waived for qualifying candidates and a work study program is available that will provide modest living expenses. A stipend ($10,700) is available for qualifying Ph.D. candidates.

Local Accommodations: The Pillars, which is adjacent to RPCI, has rates between $40.00 and $79.00 depending on the length of stay. The Holiday Inn ($71 per night) has free shuttle service to both the Buffalo Airport and RPCI. Local restaurants abound within walking distance of either RPCI or the Holiday Inn. Other hotels and rental locations (for longer visits) are available.

Transportation: Bus, Amtrak and Airline service to Buffalo is convenient and frequent. RPCI is 10 min. by taxi, limo, or subway from the bus (Oak St.) or train stations (Exchange St.) or 15 min. from the airport by taxis or limo. All major rental car agencies are at all three locations.

Cost of Internships: A basic rate of $100/day is charged for standard laboratory tests. These include standard reagents, antibodies and flow cytometry time. Costs for non standard materials or supplies if any, will be determined after discussion of the particular project requirements. All times during the year are equally good except the Thanksgiving to Christmas period.

OUTREACH PROGRAM

The Great Lakes International Imaging and Flow Cytometry Association Meeting:

There are several regional users meetings that have been successful in providing a forum for the exchange of ideas and information related to flow cytometry. These meetings are often sponsored by the companies that offer instruments and reagents. The Great Lakes region has a very high concentration of flow cytometry laboratories and this association was formed to bring together all flow cytometry users and especially their students. The meeting, held annually on the first weekend of October in Milwaukee, WI, 1999, is open to anyone. Registration is $65.00 and includes a reception Friday, breakfast, lunch and a banquet on Saturday and a breakfast on Sunday. The major focus of this meeting is to bring clinical and research applications together. Of particular importance to us will be students, post doctoral fellows and young investigators to highlight their research and provide a forum for its presentation. Click on the above link for more information.

The GLIIFCA Home Page

GREAT LAKES INTERNATIONAL IMAGING AND FLOW CYTOMETRY ASSOCIATION STEERING COMMITTEE:

CANADA
David Hedley Princess Margaret Hosp 500 Sherbourne St. Toronto, Ontario Canada M4X 1K9 phone 416-924-0671 FAX 416-413-2026 david_hedley@pmh.toronto.on.ca	Dr. Francis Mandy 3252 LCDC Building Ottawa, Ontario Canada K1A 022 phone 613-957-0174 FAX 613-957-7258 fmandy@hpb.hwc.ca

ILLINOIS
Julie Auger Univ. of Chicago 5841 S. Maryland Av. MC 1089 Chicago, IL 60637 phone 773-702-9212 fax: 773-702-3701 jauger@flowcity.bsd.uchicago.edu	Charles Goolsby Northwestern Mem. Hosp. Wesley, Room 528 250 E. Superior Chicago, IL 60611 phone 312-908-2430 fax: 312-503-8240 c-goolsby@nwu.edu
Maurice R.G. O'Gorman Director, Diagnostic Imm. Lab The Children's Memorial Hospital 2300 Children's Plaza Chicago, IL 60614 phone 773-880-3070,4361 fax: 773-880-3739 mogorman@anima.nums.nwu.edu	T. Vincent Shankey Loyola Univ. Med. Ctr. Dept. of Urology 2160 S. First Ave. Stritch School of Medicine Maywood, IL 60153 phone 708-216-5102 fax: 708-216-6585 tshanke@luc.edu
INDIANA
J. Paul Robinson Director, Purdue Univ. Cytometry Lab. 1515 Hansen Hall, B050 West Lafayette, IN 47907 phone 765-494-6449 fax: 765-494-0517 robinson@flowcyt.cyto.purdue.edu	Philip Marder Biotech. Res. /Lilly Res. Labs DC 0444 Lilly Corp. Ctr. Indianapolis, IN 46285 phone 317-276-5071 fax: 317-277-2934 marder_philip@lilly.com
IOWA Bruce Pesch National Animal Disease Center Box 70 2300 Dayton Rd Ames, IA 50010 phone: (515) 663-7626 fax: (515) 663-7458 MICHIGAN
Bruce H. Davis Dept. of Clinical Pathology William Beaumont Hospital 3601 West Thirteen Mile Road Royal Oak, MI 48073-6769 phone 810-551-5137 fax: 810-551-1262 bdavis@beaumont.edu	Alexander Nakeff Lande MRB 327 Wayne State Univ. Sch. Med. 550 E. Canfield Detroit, MI 48201 phone 313-577-7923 fax: 313-577-7925 nakeff@oncgate.roc.wayne.edu
MINNESOTA
Waclaw Jaszcz Dept. of Lab. Med. & Path. IFC Lab. UMHC Box 198 Minneapolis, MN 55455 phone 612-625-2171 fax: 612-625-0617 jaszc001@maroon.tc.umn.edu	Wendy R. Walters Methodist Hospital Labs. 6500 Excelsior Blvd. St, Louis Park, MN 55426 phone 612-932-5388 fax: 612-993-6193
NEW YORK
Carleton C. Stewart Laboratory of Flow Cytometry Roswell Park Cancer Inst. Elm and Carlton Sts. Buffalo, NY 14263 phone 716-845-8471 fax: 716-845-8806 stewart@sc3101.med.buffalo.edu	John M Lehman Albany Medical College Dept of Microbiology Albany, NY 12208 phone 518-262-5908 fax: 518-262-5748 jlehman@ccgateway.amc.edu
Zbigniew Darzynkiewicz Brander Cancer Research Institute New York Medical College 100 Grasslands Rd. Elmsford, NY, NY 10523 phone: 914-347-2801 fax: 914-347-2804 darzynk@nymc.edu
OHIO
Charles L. Hitchcock Path. Dept., The Ohio State University 129 Hamilton Hall 1645 Neil Ave. Columbus, OH 43210 phone: 614-293-8983 fax: 614-293-7072 hitchcock-1@medctr.osu.edu	James W. Jacobberger Case Western Reserve Med. Sch. Dept. of Genetics 10900 Euclid Ave. Cleveland, OH 44106-4901 phone 216-368-4645 fax: 216-368-3432 jwj@po.cwru.edu
PENNSYLVANIA
Jonni Moore Univ. of PA 203 John Morgan Bldg. 3620 Hamilton Walk Philadelphia, PA 19104-6082 phone 215-898-6853 fax:: 215-898-4227 moorej@mail.med.upenn.edu	Stanley E. Shackney Allegheny Singer Res. Inst. 320 E. North Ave. Pittsburgh, PA 15212 phone 412-359-6711 fax: 412-359-3238 shackney@asri.edu
Katharine A. Muirhead SciGro, Inc. 7 Great Valley Parkway, Suite 210 Malvern, PA 19355-1447 phone 610-889-1492 fax: 610-889-9726 kmuirhead@scigro.com	Betsy Ohlsson-Wilhelm Scigro Inc 7 Great Valley Pkwy Suite 210 Malvern PA 19355 phone: (610) 889-1492 fax: (610) 889-9726 bmow@scigro.com
WISCONSIN
Wendy S. Kopinski Med. Coll. of WI Path Bldg, 865 N 87th St Milwaukee, WI 53226 phone 414-456-7621 fax: 414-456-6305 Wendy_Kopinski@Path.Mcw.Edu	Kathleen Schell CSC-K4/535 600 Highland Ave. Madison WI 53792 phone: 608-263-0313 fax: 608-263-8613 kschell@facstaff.wisc.edu

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