Written by Rick Grazzini.
Edited and HTML by Beth Matney

This information is distributed to assist plant breeders, both laypersons and professionals.

These solutions --- the final working solutions --- are useful in attempts to induce polyploidy in plants.

My thanks to all who have helped this discussion get to this point:

• Tony Avent
• Richard Craig
• George Darrow (in spirit)
• Haig Dermen (in spirit)
• Arthur Evans
• John Fiala (in spirit)
• James Gossard (c/o KW)
• Jim Hawes
• Augie Kehr
• Kay Lancaster
• Beth Matney
• Dan Nelson
• Kevin Vaughn
• Jim Waddick
• Kevin Walek (KW)
• John Weagle

This information has been compiled from a number of sources, including USDA reprints, classroom notes, email posts, and published texts.  It has been supplemented by my experience, as well as that of the contributing personnel list above.

The bottom line is BE CAREFUL.  If you are not familiar with how to safely handle poisons --- DON'T DO IT!

TO PREPARE WORKING SOLUTIONS OF COLCHICINE

TO PREPARE STOCK SOLUTIONS OF ORYZALIN: 100 millimolar (100 mM)

TO MAKE WORKING SOLUTIONS OF ORYZALIN (10 uM and 100 uM)