Rutgers, The State University of New Jersey, New Brunswick
Ph.D. in Chemistry, 1997, M.S. in Chemistry, 1996
University of Science and Technology of China, Hefei, China
B.S. in Chemistry, 1988
EXPERIENCE: Scientist, Jan. 1999- present
Consensus Pharmaceuticals, Inc., Medford, Massachusetts.
Postdoctoral fellow, Oct. 97 - Jan. 1999
Center for Advanced Biotechnology and Medicine (CABM), University of
Medicine and Dentistry of New Jersey, Piscataway, New Jersey.
Design and Synthesis of antisense peptide-DNA conjugates for Breast
Develop new liquid-phase synthesis methodology to generate soluble peptide
In charge of lab safety issues.
Graduate Assistant, Aug. 92 - Sept. 97
Department of Chemistry, Rutgers University, New Brunswick, New Jersey.
Played key role in the AIDS drug research team.
Developed a gel shift binding assay for screening small molecules specifically
binding to DNA or RNA.
Designed, synthesized, and screened series of Tat peptide analogs and peptide-oligonuclotide
conjugates targeting TAR RNA of HIV-1.
Discovered a Tat peptide analog that can inhibit HIV-1 replication. (Patent
Engaged in setting up cell uptake assay, drug stability assay and drug
Analyzed and interpreted data in terms of pharmacokinetics and pharmacodynamics
model of drug action and its interaction.
Taught undergraduate courses in Experimental Chemistry.
Research Assistant, Sept. 91 - Aug. 92.
National Laboratory of Computer Chemistry, Chinese Academy of Sciences,
Engaged in Pesticide Database setup and SAR studies.
Research Assistant, Jan. 88 - July 91.
University of Science and Technology of China, Hefei, China.
Prepared superconducting thin films by plasma technique and characterized
the structure of corundum type oxide and superconducting oxides by IR and
Taught undergraduate courses in Physical Chemistry and Crystal Chemistry.
HONORS and AWARD:
CABM Fellowship, Rutgers University (1995).
People's scholarship, University of Science and Technology of China (1985
SKILLS: Analytical and Bioanalytical Chemistry:
HPLC purification of peptides, oligonucleotides, and small molecules.
RNA, DNA and Protein Gel Electrophoresis.
TLC, Fluorescence and UV-Vis spectrometry, ELISA.
Protein Sequencing, Amino Acid Analysis.
Developed a quantitative Gel Shift Binding Assay and measure the
dissociation constants of specific binding.
Separated organic reaction mixtures by Extraction, HPLC and Gel Filtration
Determined molecular weight of peptides and oligonucleotides by MALDI-MS.
Characterized protected amino acid derivatives by NMR.
Characterized the structure of corundum type oxide thin films by
Characterized the oxide superconductor structure by X-ray Diffraction.
Determined atomic composition of superconducting film by Atomic Absorption
Synthesized peptide and oligonucleotide by Solid-Phase Synthesis and Liquid-Phase
Expertise on conjugation reactions, peptide modifications, and PEG chemistry.
Familiar with Combinatorial Library Syntheses.
Carried out Cloning, Restriction Mapping and Analysis, plasmid isolation,
Performed RNase H Cleavage Assay and Phosphor Image Analysis.
Working knowledge of cell culture and cell uptake studies.
Windows, DOS, VMS-VAX, UNIX.
Programmed with FORTRAN, BASIC, HTML.
Expertise in Excel, Word, Photoshop, SigmaPlot, Insight II, GCG, and other
applications and database programs.
J.H. Lai and J. Wang, "Purification and Analysis of Cyclic
Peptide Libraries, and Compositions Thereof". Patent Pending.
J.H. Lai, J.J. Johnson, L. Josephson, E.K. Kolaczkowski, J. Wang,
J.J. Nestor, and S.A. Kates. "Nucleophilic and Conformational Effects of
Amino Acids in Head-to-Tail Cyclic Peptide Libraries", Proceedings of the
6th International Symposium on Solid Phase Synthesis and Combinatorial
Chemical Libraries, (R. Epton, Ed.) Mayflower Scientific Ltd., Kingswinford,
England, in press.
I. Choudhury, J. Wang, S. Stein, A.B. Rabson and M.J. Leibowitz
"Translational Effects of Peptide Antagonists of Tat Protein of HIV-1",
Journal of General Virology, 80(Pt3): 777-82 (1999).
J. Wang, S. Stein, M.J. Leibowitz and A.B. Rabson, "Inhibition of
HIV-1 Replication by a Tat RNA-binding Domain Peptide Analog", patent pending.
S-Y. Huang, S. Pooyan, J. Wang, I. Choudhury, M.J. Leibowitz and
S. Stein. "A Polyethylene Glycol Copolymer for Carrying and Releasing Multiple
Copies of Cysteine-Containing Peptides", Bioconjugate Chemistry,
9(5) 612-617 (1998).
I. Choudhury, J. Wang, A.B. Rabson, S. Pooyan, M.J. Leibowitz and
S. Stein, "Inhibition of HIV-1 Replication by a Tat RNA-binding Domain
Peptide Analog", Journal of Acquired Immune Deficiency Syndromes and
Human Retrovirology 17, 104-111 (1998).
C.E. Lau and J. Wang "Alprazolam, Caffeine and Their Interaction:
Relating DRL Performance to Pharmacokinetics". Psychopharmacology
126, 115-124 (1996).
J. Wang, S-Y. Huang, I. Choudhury, M.J. Leibowitz and S. Stein.
"Use of a Polyethylene Glycol-Peptide Conjugate in a Competition Gel Shift
Assay for Screening Potential Antagonists of HIV-1 Tat Protein Binding
to TAR RNA", Analytical Biochemistry, 232, 238-242 (1995).
C.H. Tung, J. Wang, M.J. Leibowitz and S. Stein. "Dual-specificity
Interaction of HIV-1 TAR RNA with Tat Peptide-oligonucleotide Conjugates",
Chemistry, 6(3) 292-295 (1995).
Y Qian, S Yan, J Wang, P Fu and Z Chen. "An IR Study on Thin Films
of Corundum Type Oxides". Chinese Journal of Chemical Physics, 5(4)
Z. Chen, G Zhou, Z Li, J Wang, D Sun and Y Qian. "Structure Characteristics
of High Tc Superconducting Oxide in (Bi,Pb)-Sr-Ca-Cu-O system". Molecular
Crystals &. liquid Crystals, 184, 401-408 (1990).
W liu, J Wang, Z Yang, Y He, H Zhao, Z Chen and Y Qian. "Preparation
of Superconducting Bi-Sr-Ca-Cu-O Thin Films by RF Sputtering". Chinese
Journal of Low Temperature Physics, 11(6), 464-469 (1989) (in Chinese).