This was posted on rec.games.video.sega on the USENET. I think it was a poll of the readers of a Japanese video game magazine in 1997.

1. Sakura Shinguji (Sakura Taisen)
2. Shiori Fujisaki (Tokimeki Memorial)
3. Rei Ayanami (Evangelion)
4. Pai Chan (Virtual Fighter 2)
5. Chun Li (Street Fighter Zero 2)
6. Mizuho Yuki (Kakkyuusei)
7. Morrigan (Vampire Hunter)
8. Nakoruru (Samurai Spirits 3)
9. Kyouko Misaki (Idol Mahjong Suchie Pie)
10. Mai Sakuragi (Doukyuusei ~if~)
11. Aruru Naja (Puyo Puyo)
12. Sara Bryant (Virtua Fighter 2)
13. Luna (Lunar ~Silver Star Story~)
14. Misa Tanaka (Doukyuusei ~if~)
15. Sakura (Street Fighter Zero 2)
16. Ai Minamizato (Kakkyuusei)
17. Marina Hojo (EVE burst error)
18. Yuna (Galaxy Fraulein Yuna)
19. Yuuko Asahina (Tokimeki Memorial)
20. Saki (Quiz Nanairo Dreams)
21. Hoshino Ruri (Nadeshiko)
22. Claris (NiGHTS)
23. Yuri Sakazaki (King of Fighters '96)
24. Athena Asamiya (King of Fighters '96)
25. Mizuki Tohno (Super Real Mahjong PV)
26. Mahoko Mochida (Kakkyuusei)
27. Soryu Asuka Langley (Evangelion)
28. Saki Nijino (Tokimeki Memorial)
29. Honey (Fighting Vipers)
30. Kumi Tanaka (Debut)
31. Rimururu (Samurai Spirits RPG)
32. Ellis (Toshinden S)
33. Kurara (Purikura Daisakusen)
34. Akira Hayasaka (Super Real Mahjong PV)
35. Lei-Lei (Vampire Hunter)
36. Ayako Katagiri (Tokimeki Memorial)
37. Lola (Enemy Zero)
38. Arieta (Cyberbots)
39. Miko Kamiyama (Kakkyuusei)
40. Your daughter (Princess Maker 2)
41. Iris (Sakura Taisen)
42. Miku (Metal Fighter Miku)
43. Yumimi (Yimimi Mix Remix)
44. Ryoko Inoue (Roommate ~Inoue Ryoko~)
45. King (King of Fighters '96)
46. Aya Fujiwara (Super Real Mahjong PV)
47. Koyori (Sengoku Blade)
48. Mature (King of Fighters '96)
49. Yuina Himoo (Tokimeki Memorial)
50. Ryoko Kano (Kakkyuusei)

I know, some of them are like "huh?" unless you play a lot of Japanese games. Mahjong games? But there is a Galaxy Fraulein Yuna game! Wonder if it's good. The Evangelion girls did really well, as did characters from Street Fighter (no suprise) and Pai and Sarah too. I don't think Pai beats Chun Li tho. I'm suprised Honey didn't do better. She's my favorite! Lara from Tomb Raider didnt make the list, maybe cuz it's not out there. Same goes for Arial from Wipeout. Um, Claris is kinda young. I mean, she does have big boobs, but how old is she supposta be? Here are my faves:
1. Honey/Candy from Fighters Megamix/Fighting Vipers
2. Rei Ayanami (actually I don't own any Evangelion games so it doesnt count)
3. Koyori from Sengoku Blade
4. Arial Tetsuo from Wipeout
5. Felicia from Darkstalkers
6. Chun Li from Street Fighter
7. Ellis from Battle Arena Toshinden
8. Lynn Minmay from SDF Macross
9. Cammy from Street Fighter
10. Grace from Fighting Vipers/Fighters Megamix
11. Pai Chan from Virtua Fighter/Fighters Megamix
12. Sarah from Virtua Fighter/Fighters Megamix
13. Lara Croft from Tomb Raider (actually, I think Honey has bigger boobies)
14. Nicole from Guardian Heroes
15. Sofia from Battle Arena Toshinden