Using Microscopes

<body topmargin=0 leftmargin=0 marginheight=0 marginwidth=0> <script type="text/javascript"> //OwnerIQ var __oiq_pct = 50; if( __oiq_pct>=100 || Math.floor(Math.random()*100/(100-__oiq_pct)) > 0 ) { var _oiqq = _oiqq || []; _oiqq.push(['oiq_addPageBrand','Lycos']); _oiqq.push(['oiq_addPageCat','Internet > Websites']); _oiqq.push(['oiq_addPageLifecycle','Intend']); _oiqq.push(['oiq_doTag']); (function() { var oiq = document.createElement('script'); oiq.type = 'text/javascript'; oiq.async = true; oiq.src = document.location.protocol + '//px.owneriq.net/stas/s/lycosn.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(oiq, s); })(); } //Google Analytics var _gaq = _gaq || []; _gaq.push(['_setAccount','UA-21402695-19']); _gaq.push(['_setDomainName','tripod.com']); _gaq.push(['_setCustomVar',1,'member_name','arturo_lopez_1',3]); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); //Lycos Init function getReferrer() { var all= this.document.cookie; if (all== '') return false; var cookie_name = 'REFERRER='; var start = all.lastIndexOf(cookie_name); if (start == -1) return false; start += cookie_name.length; var end = all.indexOf(';', start); if (end == -1) end = all.length; return all.substring(start, end); } function getQuery() { var rfr = getReferrer(); if (rfr == '') return false; var q = extractQuery(rfr, 'yahoo.com', 'p='); if (q) return q; q = extractQuery(rfr, '', 'q='); return q ? q : ""; } function extractQuery(full, site, q_param) { var start = full.lastIndexOf(site); if (start == -1) return false; start = full.lastIndexOf(q_param); if (start == -1) return false; start += q_param.length; var end = full.indexOf('&', start); if (end == -1) end = full.length; return unescape(full.substring(start, end)).split(" ").join("+"); } function generateHref(atag, template){ atag.href=template.replace('_MYURL_', window.location.href.replace('http://', '')).replace('_MYTITLE_','Check%20out%20this%20Tripod%20Member%20site!'); } var lycos_ad = Array(); var lycos_onload_timer; var cm_role = "live"; var cm_host = "tripod.lycos.com"; var cm_taxid = "/memberembedded"; var tripod_member_name = "arturo_lopez_1"; var tripod_member_page = "arturo_lopez_1/id280.htm"; var tripod_ratings_hash = "1728523774:aa7e193684a4878085580822485be199"; var lycos_ad_category = {"dmoz":"science\/educational_resources","ontarget":"&CAT=education","find_what":"school at home"}; var lycos_ad_remote_addr = "209.202.244.9"; var lycos_ad_www_server = "www.tripod.lycos.com"; var lycos_ad_track_small = ""; var lycos_ad_track_served = ""; var lycos_search_query = getQuery(); </script> <script type="text/javascript" src="https://scripts.lycos.com/catman/init.js"></script> <script type='text/javascript'> var googletag = googletag || {}; googletag.cmd = googletag.cmd || []; (function() { var gads = document.createElement('script'); gads.async = true; gads.type = 'text/javascript'; var useSSL = 'https:' == document.location.protocol; gads.src = (useSSL ? 'https:' : 'http:') + '//www.googletagservices.com/tag/js/gpt.js'; var node = document.getElementsByTagName('script')[0]; node.parentNode.insertBefore(gads, node); })(); </script> <script type='text/javascript'> googletag.cmd.push(function() { googletag.defineSlot('/95963596/TRI_300X250_dfp', [300, 250], 'div-gpt-ad-1450204159126-0').addService(googletag.pubads()); googletag.defineSlot('/95963596/TRI_above_728x90_dfp', [728, 90], 'div-gpt-ad-1450204159126-1').addService(googletag.pubads()); googletag.defineSlot('/95963596/TRI_below_728x90_dfp', [728, 90], 'div-gpt-ad-1450204159126-2').addService(googletag.pubads()); googletag.pubads().enableSingleRequest(); googletag.enableServices(); }); </script> <script type="text/javascript"> (function(isV) { if( !isV ) { return; } var adMgr = new AdManager(); var lycos_prod_set = adMgr.chooseProductSet(); var slots = ["leaderboard", "leaderboard2", "toolbar_image", "toolbar_text", "smallbox", "top_promo", "footer2", "slider"]; var adCat = this.lycos_ad_category; adMgr.setForcedParam('page', (adCat && adCat.dmoz) ? adCat.dmoz : 'member'); if (this.lycos_search_query) { adMgr.setForcedParam("keyword", this.lycos_search_query); } else if(adCat && adCat.find_what) { adMgr.setForcedParam('keyword', adCat.find_what); } for (var s in slots) { var slot = slots[s]; if (adMgr.isSlotAvailable(slot)) { this.lycos_ad[slot] = adMgr.getSlot(slot); } } adMgr.renderHeader(); adMgr.renderFooter(); }((function() { var w = 0, h = 0, minimumThreshold = 300; if (top == self) { return true; } if (typeof(window.innerWidth) == 'number' ) { w = window.innerWidth; h = window.innerHeight; } else if (document.documentElement && (document.documentElement.clientWidth || document.documentElement.clientHeight)) { w = document.documentElement.clientWidth; h = document.documentElement.clientHeight; } else if (document.body && (document.body.clientWidth || document.body.clientHeight)) { w = document.body.clientWidth; h = document.body.clientHeight; } return ((w > minimumThreshold) && (h > minimumThreshold)); }()))); window.onload = function() { var f = document.getElementById("FooterAd"); var b = document.getElementsByTagName("body")[0]; b.appendChild(f); f.style.display = "block"; document.getElementById('lycosFooterAdiFrame').src = '/adm/ad/footerAd.iframe.html'; // DOM Inj Ad (function(isTrellix) { var e = document.createElement('iframe'); e.style.border = '0'; e.style.margin = 0; e.style.display = 'block'; e.style.cssFloat = 'right'; e.style.height = '254px'; e.style.overflow = 'hidden'; e.style.padding = 0; e.style.width = '300px'; var isBlokedByDomain = function( href ) { var blockedDomains = [ "ananyaporn13000.tripod.com", "xxxpornxxx.tripod.com" ]; var flag = false; for( var i=0; i<blockedDomains.length; i++ ) { if( href.search( blockedDomains[ i ] ) >= 0 ) { flag = true; } } return flag; } var getMetaContent = function( metaName ) { var metas = document.getElementsByTagName('meta'); for (i=0; i<metas.length; i++) { if( metas[i].getAttribute("name") == metaName ) { return metas[i].getAttribute("content"); } } return false; } var getCommentNodes = function(regexPattern) { var nodes = {}; var nodesA = []; var preferredNodesList = ['a', 'c', 'b']; (function getNodesThatHaveComments(n, pattern) { if (n.hasChildNodes()) { if (n.tagName === 'IFRAME') { return false; } for (var i = 0; i < n.childNodes.length; i++) { if ((n.childNodes[i].nodeType === 8) && (pattern.test(n.childNodes[i].nodeValue))) { var areaName = pattern.exec(n.childNodes[i].nodeValue)[1]; nodes[areaName] = n; } else if (n.childNodes[i].nodeType === 1) { getNodesThatHaveComments(n.childNodes[i], pattern); } } } }(document.body, regexPattern)); for (var i in preferredNodesList) { if (nodes[preferredNodesList[i]]) { if( isTrellix && nodes[preferredNodesList[i]].parentNode.parentNode.parentNode.parentNode ) { nodesA.push(nodes[preferredNodesList[i]].parentNode.parentNode.parentNode.parentNode); } else { nodesA.push( nodes[preferredNodesList[i]] ); } } } return nodesA; } var properNode = null; var areaNodes = getCommentNodes( new RegExp( '^area Type="area_(\\w+)"' ) ); for (var i = 0; i < areaNodes.length; i++) { var a = parseInt(getComputedStyle(areaNodes[i]).width); if ((a >= 300) && (a <= 400)) { properNode = areaNodes[i]; break; } } var propertyName = getMetaContent("property") || false; if( isTrellix && (properNode) ) { e.src = '/adm/ad/injectAd.iframe.html'; properNode.insertBefore(e, properNode.firstChild); } else if( isTrellix && !( properNode ) ) // Slap the ad eventhought there is no alocated slot { e.src = '/adm/ad/injectAd.iframe.html'; e.style.cssFloat = 'none'; var cdiv = document.createElement('div'); cdiv.style = "width:300px;margin:10px auto;"; cdiv.appendChild( e ); b.insertBefore(cdiv, b.lastChild); } else if( !isBlokedByDomain( location.href ) ) { var injF = document.createElement('iframe'); injF.style.border = '0'; injF.style.margin = 0; injF.style.display = 'block'; injF.style.cssFloat = 'none'; injF.style.height = '254px'; injF.style.overflow = 'hidden'; injF.style.padding = 0; injF.style.width = '300px'; injF.src = '/adm/ad/injectAd.iframe.html'; if( b && ( !isTrellix || ( typeof isTrellix == "undefined" ) ) ) // All other tripod props { var cdiv = document.createElement('div'); cdiv.style = "width:300px;margin:10px auto;"; cdiv.appendChild( injF ); b.insertBefore(cdiv, b.lastChild); } } }( document.isTrellix )); } </script> <div id="tb_container" style="background:#DFDCCF; border-bottom:1px solid #393939; position:relative; z-index:999999999!important">  <style> .adCenterClass{margin:0 auto} </style> <div id="tb_ad" class="adCenterClass" style="display:block!important; overflow:hidden; width:916px;"> <div id="ad_container" style="display:block!important; float:left; width:728px "> <script type="text/javascript"> if (typeof lycos_ad !== "undefined" && "leaderboard" in lycos_ad) { document.write(lycos_ad['leaderboard']); } </script> </div> </div> </div> <script type="text/javascript"> if (typeof lycos_ad !== "undefined" && "slider" in lycos_ad) { document.write(lycos_ad['slider']); } </script>  <div id="FooterAd" style="background:#DFDCCF; border-top:1px solid #393939; clear:both; display:none; width:100%!important; position:relative; z-index:999999!important; height:90px!important"> <div class="adCenterClass" style="display:block!important; overflow:hidden; width:916px;"> <div id="footerAd_container" style="display:block!important; float:left; width:728px"> <iframe id="lycosFooterAdiFrame" style="border:0; display:block; float:left; height:96px; overflow:hidden; padding:0; width:750px"></iframe> </div> </div> </div> <table cellpadding=0 cellspacing=0 border=0><tr> <td valign="top" colspan=2 height=110 BGCOLOR="#666699" TEXT="#080000" bgproperties="fixed" background="04900840erlcjm.gif"> <div> <FONT SIZE="5" COLOR="#FFFFFF" FACE="Arial" ><B>TeachersAide!</b></FONT><B>     |     </b><A HREF="index.htm" TARGET="_top" TITLE="TeachersAide!"><U><B>home</b></U></A></div> <div> <B>Using Microscopes</b></div> <div> </div> </td> </tr><tr> <td valign="top" width=190 BGCOLOR="#CCCCFF" TEXT="#080000" background="04311c00egdysm.gif"> <div> <B>Theresa Figarelli, Jefferson Elementary, Everett, WA</b></div> <bR> <div> <B>Appropriate for Grades 3-6</b></div> </td> <td valign="top" height=370 BGCOLOR="#FFFFFF" TEXT="#080000"> <bR> <div> <B><U>Using Microscopes</U></b></div> <bR> <div> <B>Overview:</b>  This activity is used during a unit about cells.  I use it to give the students the opportunity to see cells and to discover the difference between plants and animal cells.</div> <bR> <div> <B>Purpose:</b>  To further the students understanding of cells and to give them the experience of using a microscope.</div> <bR> <div> <B>Objectives:</b>  The students will be able to:</div> <bR> <div> 1. Describe the differences between plant and animal cells.</div> <div> 2. Operate a Microscope.</div> <div> 3. Examine cheek cells, onion cells, and potato cells.</div> <bR> <div> <B>Activities:</b></div> <bR> <div> <B>1. How to Use a Microscope:</b>  The teacher will need to prepare a worksheet for the students showing a diagram of a microscope labeling all of its parts.  The teacher will then provide the students with microscopes and guide them through an introduction to the following parts from top to bottom:</div> <bR> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Eyepiece 10x</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Body tube</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Revolving nose piece</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Objective lens 4x (low):  10x (medium): 40x (high)</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Stage</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Stage clips</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Carrying arm</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Mirror or light source (lamp)</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Base</div> <bR> <div> <B>2.  Setting up a "Wet Mount Slide":</b>  The teacher explains that a wet mount slide gets it name because it is wet with either stain or water.  Stains are used to color parts of cells so they may be seen.  In order to view something with a microscope a person must be able to see through it.  The object must let light through it - this means translucent.</div> <bR> <div> The teacher then demonstrates how to make a wet mount slide.  Then the student will advance to prepare their won slides for observation.  The teach may draw the following diagram on the board and describe what she will do with the materials.</div> <bR> <div> A wet mount slide includes the following:  a slid, a cover slip, a specimen, a drop of stain or water.  When preparing a slide, hold the cover slip at an angle (as shown in the diagram above) and let it drop onto a slide slowly trapping the specimen between the two pieces of glass.  A piece of onion skin is easy to use in theis first activity.</div> <bR> <div> <B>3.  Observing and comparing cells:</b>  Depending on the age of the students the teacher may choos to read the following directions to his/her students or share them on a worksheet:</div> <bR> <div> <B>Onion Skin</b></div> <bR> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">First take a piece of onion skin off the onion.</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">put it flat on a slide.</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">bring the slide to the teacher for a drop of iodine stain.</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">carefully put on a cover slip remembering to angle it.</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">examine the cell under low then medium power.</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Ask the teacher to put it on high power.</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Draw a few cells showing what you observed in the space provided.</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Describe in a few sentences what you saw while looking at the cell through the microscope.</div> <bR> <div> <B>Cheek Cells</b></div> <bR> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Using a toothpick, scrape the inside of your mouth.</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Place it carefully on the center of the slide.</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Bring it to the leader for a drop of blue stain.</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Repeat steps from above - starting with examine the cell.</div> <bR> <div> <B>Potato Cells</b></div> <bR> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Using a knife, carefully cut a very thin slice of potato.</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Have the leader add a drop of iodine stain.</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Repeat steps from above - startin with examine the cell.</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Draw a few cells in the space provided.</div> <div> <IMG BORDER="0" SRC="Symb075c00b700f000000000.gif" HEIGHT="16" WIDTH="24" ALIGN="bottom" HSPACE="0" VSPACE="0">Describe what you saw.</div> <bR> <div> Now the teacher facilitates a discussion comparing and contrasting what the students observed.  The following vocabulary should be discussed and or defined:</div> <bR> <div> Cell Walls - Plants have thick cell walls to strengthen the plant stem.</div> <div> Cell Membranes - Animals have thin membranes because they have other forms of scheletons.</div> <div> Chloroplasts - Green colored structures that produce food.</div> <div> Nucleus - Both plants and animals have these:  they control heredity and cell division.</div> <div> Cytoplasm - A clear liquid where most of the cells life functions occur.</div> <bR> <div> After the discussion the students should have the opportunity to observe the slides again so they may observe the items discussed.</div> <bR> <div> <B>Resorces/Materials needed: </b> Microscopes, slides, coverslips, potato, onion, toothpicks, knife, iodine stain, blue stain, and worksheets.</div> <bR> <div> <B>Tying it all together:</b>  This activity has been used in cooperative learning groups consisting of students of mixed ages grades 1-5.  Also, it has been used several times in fifth grade classrooms.  The students enjoyed the experience and were able to learn the use of understanding of cells.  This is the best beginning microscope lesson I have found.  It is especially useful for the fifth grade unit on cells.  The students learn more about cells through actual observations than looking at textbook pictures.</div> <bR> </td> </tr></table></body>