|
vitro fertilization - IVF Specialists
Assisted Hatching
An 8-cell embryo in the process of assisted hatching
Holding pipette on left, hatching needle on right
As the shell (or zona pellucida) around the embryo is dissolved,
the needle is advanced to the left until a small opening is made
See more images that demonstrate assisted hatching in detail
Who should be treated with assisted hatching?
The most commonly used indications for assisted hatching with an in vitro
fertilization case are:
Age factor - Couples having IVF with the female partner's age over 37
Egg quality factor - Couples in which the female's day 3 follicle stimulating
hormone (FSH) level is elevated
Embryo quality factor - Couples having IVF with poor quality embryos
(excessive fragmentation or slow rates of cell division)
Zona factor - Couples having IVF with embryos that have a thick outer shell
(zona pellucida)
Previous failures - Couples having IVF that have had one or more previous IVF
cycles that failed
In our IVF clinic, we use assisted hatching on just about all cases - because
we think it increases the pregnancy and delivery rates.
How is assisted hatching performed?
1. The embryo is held with a specialized holding pipette.
2. A very delicate, hollow needle is used to expel an acidic solution against
the outer "shell" (zona pellucida) of the embryo.
3. A small hole is made in the shell by digesting it with the acidic solution.
4. The embryo is then washed and put back in culture in the incubator.
5. The embryo transfer procedure is done shortly after the hatching procedure.
Embryo transfer places the embryos in the woman's uterus where they will
hopefully implant and develop to result in a live birth.
Pregnancy rates
Pregnancy rates for in vitro fertilization procedures with assisted hatching
have been shown in some published studies to be higher than for IVF without
hatching. There is an improvement in implantation and pregnancy rates in our
IVF clinic with the use of assisted hatching. This is not seen in all IVF
programs.
It is possible to damage embryos with hatching and lower the pregnancy rate
in a program. Therefore, it is essential that if assisted hatching is done,
it must be expertly performed by properly trained embryologists.
The actual pregnancy and live birth rates seen in an individual IVF center
will vary according to the hatching technique used, the overall quality of
the laboratory, the skill of the individual performing the hatching, the
embryo transfer skills of the physician, and other factors.
|
