Jihong Wang

160 Main St., Apt #2, Medford, MA 02155
781-393-7939(H), 781-306-0808 x 7107(O)
URL: https://members.tripod.com/~jh_w/
E-mail: jihong_wang@ustc.edu
EDUCATION:
Rutgers, The State University of New Jersey, New Brunswick
Ph.D. in Chemistry, 1997, M.S. in Chemistry, 1996
  • GPA: 3.8/4.0
    • University of Science and Technology of China, Hefei, China
    B.S. in Chemistry, 1988
    EXPERIENCE:
    Scientist, Jan. 1999- present
    Consensus Pharmaceuticals, Inc., Medford, Massachusetts.
    Postdoctoral fellow, Oct. 97 - Jan. 1999
    Center for Advanced Biotechnology and Medicine (CABM), University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey.
  • Design and Synthesis of antisense peptide-DNA conjugates for  Breast Cancer treatment.
  • Develop new liquid-phase synthesis methodology to generate soluble peptide libraries.
  • In charge of lab safety issues.
    • Graduate Assistant, Aug. 92 - Sept. 97
    Department of Chemistry, Rutgers University, New Brunswick, New Jersey.
  • Played key role in the AIDS drug research team.
  • Developed a gel shift binding assay for screening small molecules specifically binding to DNA or RNA.
  • Designed, synthesized, and screened series of Tat peptide analogs and peptide-oligonuclotide conjugates targeting TAR RNA of HIV-1.
  • Discovered a Tat peptide analog that can inhibit HIV-1 replication. (Patent Pending)
  • Engaged in setting up cell uptake assay, drug stability assay and drug delivery system.
  • Analyzed and interpreted data in terms of pharmacokinetics and pharmacodynamics model of drug action and its interaction.
  • Taught undergraduate courses in Experimental Chemistry.
    • Research Assistant, Sept. 91 - Aug. 92.
    National Laboratory of Computer Chemistry, Chinese Academy of Sciences, Beijing, China.
  • Engaged in Pesticide Database setup and SAR studies.
    • Research Assistant, Jan. 88 - July 91.
    University of Science and Technology of China, Hefei, China.
  • Prepared superconducting thin films by plasma technique and characterized the structure of corundum type oxide and superconducting oxides by IR and X-ray diffraction.
  • Taught undergraduate courses in Physical Chemistry and Crystal Chemistry.
    • HONORS and AWARD:
  • CABM Fellowship, Rutgers University (1995).
  • People's scholarship, University of Science and Technology of China (1985 & 1987).
    • SKILLS:
    Analytical and Bioanalytical Chemistry:
  •  HPLC purification of peptides, oligonucleotides, and small molecules.
  •  RNA, DNA and Protein Gel Electrophoresis.
  •  TLC, Fluorescence and UV-Vis spectrometry, ELISA.
  •  Protein Sequencing, Amino Acid Analysis.
  •  Developed a quantitative Gel Shift Binding Assay and measure the dissociation constants of specific binding.
  •  Separated organic reaction mixtures by Extraction, HPLC and Gel Filtration Chromatography.
  •  Determined molecular weight of peptides and oligonucleotides by MALDI-MS.
  •  Characterized protected amino acid derivatives by NMR.
  •  Characterized the structure of corundum type oxide thin films by IR.
  •  Characterized the oxide superconductor structure by X-ray Diffraction.
  •  Determined atomic composition of superconducting film by Atomic Absorption Spectrometry.
    • Synthesis:
  • Synthesized peptide and oligonucleotide by Solid-Phase Synthesis and Liquid-Phase Synthesis.
  • Expertise on conjugation reactions, peptide modifications, and PEG chemistry.
  • Familiar with Combinatorial Library Syntheses.
    • Molecular Biology:
  • Carried out Cloning, Restriction Mapping and Analysis, plasmid isolation, PCR.
  • Performed RNase H Cleavage Assay and Phosphor Image Analysis.
  • Working knowledge of cell culture and cell uptake studies.
    • Computer:
  • Windows, DOS, VMS-VAX, UNIX.
  • Programmed with FORTRAN, BASIC, HTML.
  • Expertise in Excel, Word, Photoshop, SigmaPlot, Insight II, GCG, and other applications and database programs.
    • PUBLICATIONS:
    1.  J.H. Lai and J. Wang, "Purification and Analysis of Cyclic Peptide Libraries, and Compositions Thereof". Patent Pending.
    2.  J.H. Lai, J.J. Johnson, L. Josephson, E.K. Kolaczkowski, J. Wang, J.J. Nestor, and S.A. Kates. "Nucleophilic and Conformational Effects of Amino Acids in Head-to-Tail Cyclic Peptide Libraries", Proceedings of the 6th International  Symposium on Solid Phase Synthesis and Combinatorial Chemical Libraries, (R. Epton, Ed.) Mayflower Scientific Ltd., Kingswinford, England, in press.
    3. I. Choudhury, J. Wang, S. Stein, A.B. Rabson and M.J. Leibowitz "Translational Effects of Peptide Antagonists of Tat Protein of HIV-1",  Journal of General Virology, 80(Pt3): 777-82 (1999).
    4. J. Wang, S. Stein, M.J. Leibowitz and A.B. Rabson, "Inhibition of HIV-1 Replication by a Tat RNA-binding Domain Peptide Analog", patent pending.
    5. S-Y. Huang, S. Pooyan, J. Wang, I. Choudhury, M.J. Leibowitz and S. Stein. "A Polyethylene Glycol Copolymer for Carrying and Releasing Multiple Copies of Cysteine-Containing Peptides", Bioconjugate Chemistry, 9(5) 612-617 (1998).
    6. I. Choudhury, J. Wang, A.B. Rabson, S. Pooyan, M.J. Leibowitz and S. Stein, "Inhibition of HIV-1 Replication by a Tat RNA-binding Domain Peptide Analog", Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology 17, 104-111 (1998).
    7. C.E. Lau and J. Wang "Alprazolam, Caffeine and Their Interaction: Relating DRL Performance to Pharmacokinetics". Psychopharmacology 126, 115-124 (1996).
    8. J. Wang, S-Y. Huang, I. Choudhury, M.J. Leibowitz and S. Stein. "Use of a Polyethylene Glycol-Peptide Conjugate in a Competition Gel Shift Assay for Screening Potential Antagonists of HIV-1 Tat Protein Binding to TAR RNA", Analytical Biochemistry, 232, 238-242 (1995).
    9. C.H. Tung, J. Wang, M.J. Leibowitz and S. Stein. "Dual-specificity Interaction of HIV-1 TAR RNA with Tat Peptide-oligonucleotide Conjugates", Bioconjugate Chemistry, 6(3) 292-295 (1995).
    10. Y Qian, S Yan, J Wang, P Fu and Z Chen. "An IR Study on Thin Films of Corundum Type Oxides". Chinese Journal of Chemical Physics, 5(4) 275-280 (1992).
    11. Z. Chen, G Zhou, Z Li, J Wang, D Sun and Y Qian. "Structure Characteristics of High Tc Superconducting Oxide in (Bi,Pb)-Sr-Ca-Cu-O system". Molecular Crystals &. liquid Crystals, 184, 401-408 (1990).
    12. W liu, J Wang, Z Yang, Y He, H Zhao, Z Chen and Y Qian. "Preparation of Superconducting Bi-Sr-Ca-Cu-O Thin Films by RF Sputtering". Chinese Journal of Low Temperature Physics, 11(6), 464-469 (1989) (in Chinese).
    IMMIGRATION STATUS:
    Permanent resident of the US.