EXPERIENCE IN LABORATORY TECHNIQUES:

*  Mammalian primary and established cell culture techniques
*  Virus isolation & purification, growth curve, titration, plaque assay, neutralization test
*  Solid-phase, cell-binding and competitive RIAs ;  Indirect, avidin-biotin and competitive ELISAs
*  Fluorescence-activated cell sorter analysis, Immunofluorescence assay
*  SDS-PAGE, Western blot, Dot immunoassay, Immunoprecipitation
*  Hybridoma technique, Ascites production, Immunoaffinity chromatography
*  Interferon-gamma assay; In vitro cytotoxicity (CTL) assay
*  Solid-phase peptide synthesis; Preparative HPLC
*  Hemagglutination & its inhibition; Agar gel immunodiffusion, Counterimmunoelectrophoresis
*  Laboratory animal handling, inoculation and specimen collection procedures
*  Basic molecular biology techniques

Nagendra R. Hegde, Phone: (503) 977-2745, Email: hegden@ohsu.edu
URL: https://members.tripod.com/~n_hegde